Ms Pelosi will lead a delegation from US Congress for talks with officials.
She will be the most senior member of Congress to visit Syria since relations between the two countries soured.
Washington withdrew its ambassador to Damascus two years ago after the murder of former Lebanese PM Rafik Hariri, in which Syria denies any involvement.
The BBC's Vanessa Heaney in Washington says Nancy Pelosi's second trip to the Middle East this year shows she has no intention of letting President Bush dominate foreign policy."
This is all the dems are good for. Bowing to an enemy, and it's hilarious that Pelosi would select a certified women-hating nation to drop to her knees for. Wonder if she'll be forced to wear a burqa...